Journal
BIOFOULING
Volume 27, Issue 5, Pages 519-528Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2011.586127
Keywords
Bacillus pumilus; Bacillus indicus; anti-biofilm; 4-phenylbutanoic acid; hydrophobicity index; EPS production
Funding
- Department of Biotechnology (DBT), the Government of India (GOI) [BT/PR11994/ndb/52/134/2009, BT/BI/25/001/2006]
- Alagappa University
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Biofilm formation is a critical problem in nosocomial infections and in the aquaculture industries and biofilms show high resistance to antibiotics. The aim of the present study was to reveal a novel anti-biofilm compound from marine bacteria against antibiotic resistant Gram-positive and Gram-negative biofilms. The bacterial extract (50 mu g ml(-1)) of S6-01 (Bacillus indicus = MTCC 5559) showed 80-90% biofilm inhibition against Escherichia coli, Shigella flexneri, Proteus mirabilis and S6-15 (Bacillus pumilus MTCC 5560) showed 80-95% biofilm inhibition against all the 10 tested organisms. Furthermore, they also reduced the hydrophobicity index and extracellular polymeric substances (EPS) production. Structural elucidation of the active principle in S6-15 using GC-MS, H-1 NMR, and C-13 NMR spectral data revealed it to be 4-phenylbutanoic acid. This is the first report of 4-phenylbutanoic acid as a natural product. The purified compound (10-15 mu g ml(-1)) showed potential activity against a wide range of biofilms. This study for the first time, reports a novel anti-biofilm compound from a marine bacterium with wide application in medicine and the aquaculture industry.
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