Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 303, Issue 4, Pages 627-640Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/jmbi.2000.4152
Keywords
homology; protein evolution; pathway evolution; metabolism; TIM barrels
Categories
Ask authors/readers for more resources
We provide statistically reliable sequence evidence indicating that at least 12 of 23 SCOP (beta alpha)(8) (TIM) barrel superfamilies share a common origin. This includes all but one of the known and predicted TIM barrels found in central metabolism. The statistical evidence is complemented by an examination of the details of protein structure, with certain structural locations favouring catalytic residues even though the nature of their molecular function may change. The combined analysis of sequence, structure and function also enables us to propose a phylogeny of TIM barrels. Based on these data, we are able to examine differing theories of pathway and enzyme evolution, by mapping known TIM barrel folds to the pathways of central metabolism. The results favour widespread recruitment of enzymes between pathways, rather than a backwards evolution model, and support the idea that modern proteins may have arisen from common ancestors that bound key metabolites. (C) 2000 Academic Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available