Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 97, Issue 23, Pages 12828-12833Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.230438697
Keywords
-
Categories
Ask authors/readers for more resources
We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a kiss and run mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During kiss and run. the vesicle interior may be exposed very transiently(<6 ms), or a special configuration of the fusion pore may prevent dye exchange. We estimate that about 20% of the vesicles normally use this kiss and run pathway, and that the fraction of kiss and run events can be increased to over 80% by superfusing the synapses with hypertonic solution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available