Journal
NATURE
Volume 408, Issue 6809, Pages 225-229Publisher
MACMILLAN PUBLISHERS LTD
DOI: 10.1038/35041603
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High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway(1). After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex(2-4). Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.
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