Journal
BIOFACTORS
Volume 40, Issue 6, Pages 636-645Publisher
WILEY
DOI: 10.1002/biof.1191
Keywords
morusin; alcohol extract; HPLC-DAD; mulberry; NF-B
Funding
- earmarked fund for China Agriculture Research System (CARS) [CARS-22-ZJ0504]
- Priority Academic Program Development of Jiangsu Higher Education Institutions, People's Republic of China
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In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H-22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF- B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF- B genes. (c) 2014 BioFactors, 40(6):636-645, 2014
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