Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 529, Issue 1, Pages 171-188Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1111/j.1469-7793.2000.00171.x
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- NHLBI NIH HHS [R01HL57929, R01 HL057929, HL59614] Funding Source: Medline
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1. The mechanisms underlying electro-mechanical alternans caused by faster heart rates were investigated in perfused guinea-pig hearts stained with RH237 and Rhod-2AM to simultaneously map optical action potentials (APs) and intracellular free Ca2+ (Ca-i(2+)). 2. Fluorescence images of the heart were focused on two 16 x 16 photodiode arrays to map Ca-i(2+) (emission wavelength (lambda (em)) = 585 +/- 20 nm) and APs (lambda (em) > 715 nm) from 252 sites. Spatial resolution was 0.8 mm x 0.8 mm per diode and temporal resolution 4000 frames s(-1). 3. The mean time-to-peak for APs and [Ca2+](i), was spatially homogeneous (8.8 +/- 0.5 and 25.6 +/- 5.0 ms, respectively; n = 6). The durations of APs (APDs) and Ca-i(2+) transients were shorter at the apex and progressively longer towards the base, indicating a gradient of ventricular relaxation. 4. Restitution kinetics revealed increasingly longer delays between AP and Ca-i(2+) upstrokes (9.5 +/- 0 4 to 11.3 +/- 0.4 ms) with increasingly shorter S1-S2 intervals, particularly at the base, despite nearly normal peak [Ca2+](i). 5. Alternans of APs and Ca-i(2+) transients were induced by a decrease in cycle length (CL), if the shorter CL captured at the pacing site and was shorter than refractory periods (RPs) near the base, creating heterogeneities of conduction velocity. 6. Rate-induced alternans in normoxic hearts were concordant (long APD with large [Ca2+](i)) across the epicardium, with a magnitude (difference between odd-even signals) that varied with the local RP. Alternans were initiated by gradients of RP, producing alternans of conduction that terminated spontaneously without progressing to fibrillation.
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