Journal
ANALYTICAL CHEMISTRY
Volume 72, Issue 22, Pages 5655-5658Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac000811l
Keywords
-
Categories
Funding
- NCRR NIH HHS [RR00862] Funding Source: Medline
Ask authors/readers for more resources
Recent breakthroughs in the high-resolution structural elucidation of ion channels and transporters are prompting a growing interest in methods for characterizing integral membrane proteins. These methods are proving extremely valuable in facilitating the production of X-ray diffraction-grade crystals. Here we present a robust and straightforward mass spectrometric procedure that utilizes matrix-assisted laser desorption/ionization to analyze integral membrane proteins in the presence of detergents. The utility of this method is illustrated with examples of high-quality mass spectral data obtained from membrane proteins for which atomic resolution structural studies are ongoing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available