Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 27, Issue 9, Pages 639-645Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(00)00196-4
Keywords
human cytomegalovirus; quartz crystal microbalance; immobilization; self-assembled monolayers
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A piezoelectric affinity sensor has been developed to detect distinctive antigens of the human cytomegalovirus. Either the specific antibodies or the antigen were immobilized on the gold electrode. To develop a rapid immunoassay, various assay formats were tested in relation with the different antigen composition. First, a direct assay was carried out immobilizing the specific antibody on the crystal surface by passive adsorption. Next, Protein A, thiol/poly L-lysine mixed self-assembled monolayers were tested as methods of gold modification. A competitive format was exploited by immobilization of the antigen onto the crystal activated by SAM and poly L-lysine. This procedure yielded a preliminary calibration curve. A linear range between 2.5 and 5 mug/ml of gB epitope in solution and a detection limit of 1 mug/ml were measured. (C) 2000 Elsevier Science Inc. All rights reserved.
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