Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 275, Issue 46, Pages 36269-36277Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M006597200
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- Diabetes UK [13/0004672] Funding Source: Medline
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Increases in glucose concentration control the transcription of the preproinsulin (PPI) gene and several other genes in the pancreatic islet beta -cell. Although recent data have demonstrated that secreted insulin may regulate the PPT gene (Leibiger, I. B., Leibiger, B., Moede, T,, and Berggren, P, O, (1998) Mel. Cell 1, 933-938), the role of insulin in the control of other beta -cell genes is unexplored. To study the importance of insulin secretion in the regulation of the PPI and liver-type pyruvate kinase (L-PK) genes by glucose, we have used intranuclear microinjection of promoter-luciferase constructs into MING beta -cells and photon-counting imaging. The activity of each promoter was increased either by 30 (versus 3) mM glucose or by 1-20 nM insulin. These effects of insulin were not due to enhanced glucose metabolism since culture with the hormone had no impact on the stimulation of increases in intracellular ATP concentration caused by 30 mM glucose. Furthermore, the islet-specific glucokinase promoter and cellular glucokinase immunoreactivity were unaffected by 30 mM glucose or 20 nM insulin. Inhibition of insulin secretion with the Ca2+ channel blocker verapamil, the ATP-sensitive K+ channel opener diazoxide, or the alpha (2)-adrenergic agonist clonidine blocked the effects of glucose on L-PK gene transcription. Similarly, 30 mM glucose failed to induce the promoter after inhibition of phosphatidylinositol 3'-kinase activity with LY294002 and the expression of dominant negative-acting phosphatidylinositol 3'-kinase (Delta p85) or the phosphoinositide 3'-phosphatase PTEN (phosphatase and tensin homologue), LY294002 also diminished the activation of the L-PK gene caused by inhibition of 5'-AMP-activated protein kinase with anti-5'-AMP-activated protein kinase alpha2 antibodies. Conversely, stimulation of insulin secretion with 13 mM KCI or 10 muM tolbutamide strongly activated the PPI and L-PK promoters. These data indicate that, in MING beta -cells, stimulation of insulin secretion is important for the activation by glucose of L-PK as well as the PPI promoter, but does not cause increases in glucokinase gene expression or glucose metabolism.
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