Journal
FEBS LETTERS
Volume 485, Issue 2-3, Pages 200-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02225-0
Keywords
hydrogenase; H-2-forming; methylenetetrahydromethanopterin dehydrogenase; methanogenic archaea; Methanobacterium thermoautotrophicum; Methanococcus jannaschii; Methanopyrus kandleri
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The hmd gene, which encodes the metal-free hydrogenase in methanogenic archaea, was heterologously expressed in Escherichia coli, The overproduced enzyme was completely inactive. High activity could, however, be induced by the addition of ultrafiltrate from active enzyme denatured in 8 M urea. The active fraction in the ultrafiltrate was heat-labile and migrated on gel filtration columns with an apparent molecular mass well below 1000 Da, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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