Journal
BIOESSAYS
Volume 32, Issue 5, Pages 392-400Publisher
WILEY
DOI: 10.1002/bies.200900160
Keywords
cancer; cell lines; limitations; p53
Categories
Funding
- Foundation of Stars
- Canada Research Chair
- Canadian Institutes of Health Research
- CancerCare Manitoba Foundation
- Leukemia and Lymphoma Society of Canada
Ask authors/readers for more resources
p53 has a determinant role in cancer prevention and is among the most studied proteins in the world. The majority of studies devoted to this protein are carried out in cell lines because they are easy to use and have naturally emerged as the main research tool in laboratories. However, the p53 pathway is commonly deregulated in cancer cells, from which the experimental cell lines are generally derived. The fact that the pathway is deregulated challenges the relevance of using cancer-derived cell lines to study wildtype p53 activities, or, in a broader sense, to study any normal cellular process. In the present article, we identify and discuss a number of limitations of cell lines using examples related to p53. Finally, we point out the general limitations of cell lines and propose solutions as alternatives to these cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available