Journal
BRITISH JOURNAL OF NUTRITION
Volume 84, Issue -, Pages S245-S248Publisher
C A B INTERNATIONAL
DOI: 10.1079/096582197388626
Keywords
sucrase-isomaltase; lactase-phlorizin hydrolase; transcription; dietary carbohydrates
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To explore the underlying molecular mechanism whereby nutrients modulate the expression of intestinal digestion/absorption-related genes, we have cloned the 5' flanking regions of two representing disaccharidase genes, i.e. sucrase-isomaltase (ST) and lactase-phlorizin hydrolase (LPH), and investigated whether the binding activity of putative common nuclear factor(s) binding to the cia-elements located in these regions is altered by dietary manipulations. Orogastric feeding of a sucrose-containing diet to rats caused parallel increases in SI mRNA and LPH mRNA levels within 3 h. Among the monosaccharides tested, fructose gave rise to the most prominent increase in the mRNA levels of SI and LPH genes, which were accompanied by a coordinate rise in the mRNA levels of two microvillar hexose transporters, i.e. SGLT1 and GLUTS. Nuclear run-on assays revealed that fructose, but not glucose, increased the transcription of SI, LPH and GLUTS. DNase I footprinting analysis of the rat LPH gene showed that the protected region conserved the same sequence as the cia-element (CE-LPH1) reported in the pig LPH gene. Electrophoretic mobility shift assay using CE-LPH1 and the related cia-element of SI gene (SIF1) revealed that nuclear extracts from the jejunum of rats fed the high-starch diet gave greater density of retarded bands than those of rats fed the low-starch diet. Force feeding a fructose diet gave rise to an increase in the binding of the dimeric nuclear protein (Cdx-2) to the SIF1 element. These results suggest that the cis-elements of CE-LPH1 and SLF1 might be involved in the carbohydrate-induced increases of the transcription of LPH and SI, presumably through a change in the expression and/or binding activity of Cdx-2.
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