Journal
BIOELECTROMAGNETICS
Volume 30, Issue 7, Pages 545-555Publisher
WILEY
DOI: 10.1002/bem.20511
Keywords
large gradient high magnetic field; superconducting magnet; diamagnetic levitation; simulated gravity environment; microtubule actin crosslinking factor 1
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Funding
- National Natural Science Foundation of China [30840030]
- National High-Tech R&D Program (863) of China [2008AA12A220, 2008AA12A218]
- NPU Foundation for Fundamental Research
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The intense inhomogeneous magnetic fields acting oil the diamagnetic materials naturally present in cells can generate strong magnetic forces. We have developed a superconducting magnet platform with large gradient high magnetic field (LG-HMF), which can produce three magnetic force fields of - 1360, 0, and 1312 T-2/m, and three corresponding apparent gravity levels, namely 0, 1, and 2-g for diamagnetic materials. In this study, the effects of different magnetic force fields on osteoblast-like cells (MG-63 and MC3T3-E1) viability, microtubule actin crosslinking factor 1 (MACF1) expression and its association with cytoskeleton were investigated. Results showed that cell viability increased to different degrees after exposure to 0 or 1-g conditions for 24 h, but it decreased by about 30% under 2-g conditions compared with control conditions. An increase in MACF1 expression at the RNA or protein level was observed in osteoblast-like cells under the magnetic force field of - 1360 T-2/m (0-g) relative to 1312 T-2/m (2-g). Under control conditions, anti-MACF1 staining was scattered in the cytoplasm and partially colocalized with actin filaments (AFs) or microtubules (MTs) in the majority of osteoblast-like cells. Under 0-g conditions, MACF1 labeling was concentrated at perinuclear region and colocalization was not apparent. The patterns of anti-MACF1 labeling oil MTs varied with MTs' changing under LG-HMF environment. In conclusion, LG-HMF affects osteoblast-like cell viability, MACF1 distribution, expression, and its association with cytoskeleton to some extent. Bioelectromagnetics 30:545-555, 2009. (C) 2009 Wiley-Liss. Inc.
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