Journal
JOURNAL OF BIOTECHNOLOGY
Volume 216, Issue -, Pages 169-177Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2015.10.003
Keywords
Biodiesel; C/N ratio; Chlorella; Fatty acid composition; Heterotrophic growth; Microalgae
Categories
Funding
- Energy Policy and Planning Office (EPPO), Ministry of Energy, Thailand [060/2555]
- National Research Council of Thailand (NRCT) [WU57102]
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Chlorella sp. TISTR 8990 was cultivated heterotrophically in media with various initial carbon-to-nitrogen ratios (C/N ratio) and at different agitation speeds. The production of the biomass, its total fatty acid content and the composition of the fatty acids were affected by the C/N ratio, but not by agitation speed in the range examined. The biomass production was maximized at a C/N mass ratio of 29:1. At this C/N ratio, the biomass productivity was 0.68 g L-1 d(-1), or nearly 1.6-fold the best attainable productivity in photoautotrophic growth. The biomass yield coefficient on glucose was 0.62 g g(-1) during exponential growth. The total fatty acids (TFAs) in the freeze-dried biomass were maximum (459 mg g(-1)) at a C/N ratio of 95:1. Lower values of the C/N ratio reduced the fatty acid content of the biomass. The maximum productivity of TFAs (186 mg L-1 d(-1)) occurred at C/N ratios of 63:1 and higher. At these conditions, the fatty acids were mostly of the polyunsaturated type. Allowing the alga to remain in the stationary phase for a prolonged period after N-depletion, reduced the level of monounsaturated fatty acids and the level of polyunsaturated fatty acids increased. Biotin supplementation of the culture medium reduced the biomass productivity relative to biotin-free control, but had no effect on the total fatty acid content of the biomass. (C) 2015 Elsevier B.V. All rights reserved.
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