Journal
BIOELECTROCHEMISTRY
Volume 91, Issue -, Pages 44-51Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2013.01.001
Keywords
2,2 '-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid); Carbon nanotube; Biosensor; Dopamine; Uric acid
Funding
- National Science Council of Taiwan [97-2221-E-006-170, 99-2221-E-006-257-MY3]
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An 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)-immobilized carbon nanotube (CNT) electrode was used to simultaneously detect dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA) with differential pulse voltammetty. When ABTS was immobilized onto the CNT electrode in the presence of DA, UA and 100 mu M AA, the sensitivity to DA increased from 0.600 (+/- 0.013) to 1334 (+/- 0.010) mu A/mu M in the concentration ranges of 0.90-10 mu M and 1.87-20 mu M, respectively, and the sensitivity to UA increased from 0.030 (+/- 0.005) to 0.078 (+/- 0.006) mu A/mu M in the concentration ranges of 2.16-240 mu M and 3.07-400 mu M, respectively. These findings demonstrate that the ABTS-immobilized CNT electrode attained a higher sensitivity to UA and also a wider linear range of concentrations. (C) 2013 Elsevier B.V. All rights reserved.
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