Journal
BIOELECTROCHEMISTRY
Volume 90, Issue -, Pages 18-23Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2012.10.005
Keywords
TiO2-graphene nanocomposite; Gold nanoparticles; Lable-free; alpha-fetoprotein; Immunosensor
Funding
- National Natural Science Foundation of China [21075106]
- Program for Science & Technology Innovation Talents in Universities of Henan Province [2010HASTIT025]
- Excellent Youth Foundation of He'nan Scientific Committee [104100510020]
Ask authors/readers for more resources
A simple label-free amperometric immunosensor for protein detection is developed based on TiO2-graphene (TiO2-Gr), chitosan and gold nanoparticles (AuNPs) composite film modified glassy carbon electrode (GCE). The negatively charged AuNPs can be adsorbed on the positively charged chitosan/TiO2-Gr composite film by electrostatic adsorption, and then is used to immobilize alpha-fetoprotein antibody for the assay of alpha-fetoprotein (AFP). The interaction of antigen and antibody on the electrode interface makes a barrier for electrons and inhibits the electro-transfer, resulting in the decreased DPV signals of probe Fe(CN)(6)(3-/4-). Using this strategy, a wide detection range (0.1-300 ng mL(-1)) with the correlation coefficients of 0.992-0.994 for model target AFP is obtained. The limit of detection is 0.03 ng mL(-1) at a signal-to-noise ratio of 3. The results prove that the sensing strategy possesses sensitivity, selectivity, stability and generality, and it may be used to immobilize other biomoleculars to develop biosensor for the detection of other antigens or biocompounds. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available