Journal
BIOLOGY OF REPRODUCTION
Volume 63, Issue 6, Pages 1610-1616Publisher
SOC STUDY REPRODUCTION
DOI: 10.1095/biolreprod63.6.1610
Keywords
gametogenesis; oocyte development
Categories
Funding
- NICHD NIH HHS [HDD 22732] Funding Source: Medline
Ask authors/readers for more resources
M-Phase promoting factor (MPF) is a complex of p34(cdc2) and cyclin B. Results of previous studies in which relative mass amounts of these cell cycle regulators were determined suggested that the accumulation of p34(cdc2), rather than cyclin B, could be a limiting factor in the acquisition of meiotic competence in mouse oocytes. Nevertheless, in the absence of measurements of the absolute amount of these components of MPF, it is possible that the molar amount of p34(cdc2) is in excess to that of cyclin B, i.e., the accumulation of p34(cdc2) iS not a limiting factor. We report measurements of the absolute mass of p34(cdc2) and cyclin pi, as well as the two proximal regulators of MPF, namely cdc25C and wee1, in meiotically incompetent and competent mouse oocytes. We find that the numbers of molecules of p34(cdc2), cyclin B1, cdc25C, and wee1 in meiotically incompetent oocytes are 1.4 x 10(6) 11.3 x 10(6) 24.6 x 10(6), 15.6 x 10(6), respectively, and in meiotically competent oocytes the numbers are 14.3 X 10(6), 95.5 X 10(6), 80.0 X 10(6), 40.1 X 10(6) respectively. Thus, the concentration of cyclin pi is always in excess to that of p34(cdc2), and this is consistent with the hypothesis that the accumulation of p34(cdc2) plays a role in the acquisition of meiotic competence. Last, the concentration of cdc25C is greater than that of wee1 and the concentration of each is greater than that of p34(cdc2) in both meiotically incompetent and competent oocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available