Journal
BIOELECTROCHEMISTRY
Volume 77, Issue 2, Pages 75-81Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.06.014
Keywords
Sol-gel; Zinc oxide; Impedimetric immunosensor; Mycotoxin; Ochratoxin-A
Funding
- Department of Science & Technology (DST), Govt. of India
- Department of Science and Technology (DST) [DST/TSG/ME/2008/18, GAP-0709321, OLP-070632D]
- India-Japan project [DST/INT/JAP/P21/07]
- Department of Biotechnology, Govt. of India [DBT/GAP070832]
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Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as similar to 5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01 nM/dm(3), detection limit of 0.006 nM/dm(3), response time as 25 s and sensitivity of 189 Omega/nM/dm(3) cm(-2) with a regression coefficient of 0.997. (c) 2009 Elsevier B.V. All rights reserved.
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