Journal
BIOELECTROCHEMISTRY
Volume 76, Issue 1-2, Pages 169-174Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.05.001
Keywords
Conducting polymers; Microbial biosensors; Electrochemical polymerization
Funding
- METU
- Ege University
- TUBA
Ask authors/readers for more resources
Two types of bacterial biosensor were constructed by immobilization of Gluconobacter oxydans and Pseudomonas fluorescens cells on graphite electrodes modified with the conducting polymer; poly(1-(4-nitrophenyl)-2,5-di(2-thienyl)-1 H-pyrrole) [SNS(NO2)]. The measurement was based on the respiratory activity of cells estimated by the oxygen consumption at -0.7 V due to the metabolic activity in the presence of substrate. As well as analytical characterization, the linear detection ranges, effects of electropolymerization time, pH and cell amount were examined by using glucose as the substrate. The linear relationships were observed in the range of 0.25-4.0 mM and 0.2-1.0 mM for G. oxydans and P. fluorescens based sensors, respectively. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available