Journal
BIOELECTROCHEMISTRY
Volume 76, Issue 1-2, Pages 135-140Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.03.004
Keywords
Cellulose acetate; Graphite composite; Electrochemical sensor; Enzyme biosensor
Funding
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BD/27864/2006, PTDC/QUI/65255/2006, PTDC/QUI/65732/2006]
- POCI (co-financed by the European Community Fund FEDER)
- CEMUC(R) (Research Unit 285)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/27864/2006] Funding Source: FCT
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A new carbon-based conducting composite has been developed as electrochemical sensor and biosensor for the amperometric detection of ascorbate and glucose. Electrocatalytic oxidation of ascorbate has been done successfully at unmodified cellulose acetate-graphite composite electrodes, the sensor being highly sensitive. selective and with a low detection limit at 0.0 V vs. SCE and was successfully applied for ascorbate determination in commercial fruit juice samples. An interference free glucose biosensor has also been developed, based on the immobilisation of glucose oxidase by cross-linking with glutaraldehyde on poly (neutral red) modified composite electrodes. The biosensor exhibits a higher sensitivity of 31.5 +/- 1.7 mu A cm(-2) mM(-1) than other carbon-composite-based glucose biosensors, a detection limit of 20.3 mu M and a very short response time. (C) 2009 Elsevier B.V. All rights reserved.
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