Journal
ANIMAL GENETICS
Volume 31, Issue 6, Pages 396-399Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2052.2000.00684.x
Keywords
bovine myostatin gene; double-muscling phenotype; multiplex oligonucleotide ligation assay
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We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
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