Journal
BIOELECTROCHEMISTRY
Volume 74, Issue 2, Pages 301-305Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2008.11.002
Keywords
Nitric oxide; Modified electrode; Poly (p-phenylenevinylene) derivative; Sensor; Rat heart cells
Funding
- National Natural Science Foundation of China [30770549]
- Chenguang plan of Wuhan [200750731285]
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A novel nitric oxide (NO) electrochemical sensor was fabricated by electropolymerization with two-pulse potential method in aqueous solution containing poly (p-phenylenevinylene) derivative. The experimental results showed this sensor exhibits apparent current response to NO by cyclic voltammetry. Two anodic peaks potential are significantly shifted negatively compared with bare glass carbon electrode (GCE). Chronocoulometry were used to further validate the electron transfer process of NO oxidization on this sensor. The results indicated that oxidation of NO proceeds in two steps. The first step leading to NO2- involves one-electron transfer process and the second step corresponding to the oxidation of NO2- to NO3- involves two-electron transfer process. The amperometric response is linear with NO concentration ranging from 1.8 x 10(-7) to 1.0 x 10(-4) mol L-1 with a linear coefficient of 0.9993. The detection limit of NO is 2.3 x 10(-8) mol L-1 (S/N=3). After possible interferences were tested. NO release from the rat heart cells stimulated by L-arginine was measured using this sensor. The result is satisfactory. (C) 2008 Elsevier B.V. All rights reserved.
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