Journal
NEUROLOGIA MEDICO-CHIRURGICA
Volume 40, Issue 12, Pages 618-622Publisher
JAPAN NEUROSURGICAL SOC
DOI: 10.2176/nmc.40.618
Keywords
in vivo gene transfer; central nervous system; electroporation; green fluorescence protein; periventricular region; rats
Categories
Ask authors/readers for more resources
Gene transfer by electroporation was attempted in the normal rat brain. The reporter gene pEGFP-C1 (25 mug/5 mul) was injected into the striatum of young adult rats and various square electrical impulses were applied using a pair of electrodes implanted in the striatum. The brains were removed and sliced after 5 days. Histological examination revealed that the high energy impulses caused extensive tissue damage whereas lower energy impulses (200-400 mJ) resulted in the transfection of more than 300 cells per brain, which were widely distributed in the subependymal region of the lateral ventricle and extended long processes into the striatum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available