Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 34, Issue 2, Pages 129-137Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-6496(00)00084-2
Keywords
afipia; amoebal co-culture; alpha proteobacteria; water
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As water is a source of nosocomial infections in hospitals, the presence of fastidious Gram-negative bacteria in water samples taken in a university hospital was investigated. Water samples were inoculated onto agar plates and into amoebal microplates for co-culture. Sixty-eight a proteobacteria isolates were obtained and characterized using phenotypic methods and 16S rRNA gene sequence comparison. The latter approach divided the strains into seven clusters. Of these, one corresponded to previously recognized Afipia felis and it is likely that six were closely related new species. As these bacteria are fastidious and can not be cultivated on standard microbiological media, their possible role in hospital-acquired human infections should be investigated. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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