Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 12, Pages 5383-5386Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.12.5383-5386.2000
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An Escherichia coli strain that accumulated Ni(II) was constructed by introducing the nixA gene (coding for a nickel transport system) from Helicobacter pylori into JM109 cells that expressed a glutathione S-transferase-pea metallothionein fusion protein. The resulting strain accumulated 15 mu mol of Ni(II) per g (dry weight) from a 10 muM NI(II) solution, four times the level taken up by JM109 cells. NI(II) accumulation did not require an energy source, was inhibited by only 50% by 0.1 M NaCl, and occurred over the pH range from 3 to 9.
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