Journal
MOLECULAR CELL
Volume 6, Issue 6, Pages 1343-1354Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(00)00132-5
Keywords
-
Categories
Funding
- NCI NIH HHS [CA79801] Funding Source: Medline
- NIAMS NIH HHS [AR43768] Funding Source: Medline
Ask authors/readers for more resources
Functional proteomics provides a powerful method for monitoring global molecular responses following activation of signal transduction pathways, reporting altered protein posttranslational modification and expression. Here we combine functional proteomics with selective activation and inhibition of MKK1/2, in order to identify cellular targets regulated by the MKK/ERK cascade. Twenty-five targets of this signaling pathway were identified, of which only five were previously characterized as MKK/ERK effecters. The remaining targets suggest novel roles for this signaling cascade in cellular processes of nuclear transport, nucleotide excision repair, nucleosome assembly, membrane trafficking, and cytoskeletal regulation. This study represents an application of functional proteomics toward identifying regulated targets of a discrete signal transduction pathway and demonstrates the utility of this discovery-based strategy in elucidating novel MAP kinase pathway effecters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available