Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 119, Issue 2, Pages 165-171Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2014.07.013
Keywords
Squalene synthase; Mevalonate pathway; Triterpenoid; Carotenoid; Bacterial production
Funding
- PRESTO (JST)
- MEXT/JSPS [23108507]
- JSPS
- Mishima-Kaiun Memorial Foundation
- Futaba Electronics Memorial Foundation
- Shorai Foundation for Science and Technology
Ask authors/readers for more resources
Squalene is a precursor of thousands of bioactive triterpenoids and also has industrial value as a lubricant, health-promoting agent, and/or drop-in biofuel. To establish an efficient Escherichia coil-based system for squalene production, we tested two different squalene synthases and their mutants in combination with precursor pathways. By co-expressing a chimeric mevalonate pathway with human or Thermosynechococcus squalene synthase, E. coli accumulated squalene up to 230 mg/L or 55 mg/g-DCW in flask culture. We also determined that a significant truncation of squalene synthase at the C-terminus retains partial cellular activity. The squalene-producing strain described herein represents a convenient platform for gene discovery and the construction of the pathway toward natural and non-natural hopanoids/steroids. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available