Journal
JOURNAL OF CHEMICAL NEUROANATOMY
Volume 20, Issue 3-4, Pages 349-374Publisher
ELSEVIER
DOI: 10.1016/S0891-0618(00)00097-1
Keywords
mRNA localization; cAMP-specific phosphodiesterase; PDE4 inhibitor; radioligand autoradiography
Categories
Ask authors/readers for more resources
We have examined the distribution of Four different cyclic AMP-specific phosphodiesterase isozyme (PDE4A, PDE4B. PDE4C and PDE4D) mRNAs in the brain of different species by in situ hybridization histochemistry and by autoradiography with [H-3]rolipram. We have compared the localization of each isozyme in human brain with that in rat and monkey brain. We have Found that the four PDE4 isoforms display a differential expression pattern at both regional and cellular level in the three species. PDE4A. PDE4B and PDE4D are widely distributed in human brain, with the two latter appearing more abundant. In contrast, PDE4C in human brain, presents a more restricted distribution, limited to cortex, some thalamic nuclei and cerebellum. This is at variance with the distribution of PDE4C in rat brain, where it is found exclusively in olfactory bulb. In monkey brain, the highest expression for this isoform is found in the claustrum, and at lower levels in cortical areas and cerebellum PDE4B presented a broad distribution, being expressed in both neuronal and non neuronal cell populations. In general, the distribution of binding sites visualized with [H-3]rolipram correlated well with the expression of each PDE4 isozyme. (C) 2000 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available