Journal
INSECT MOLECULAR BIOLOGY
Volume 9, Issue 6, Pages 641-645Publisher
WILEY
DOI: 10.1046/j.1365-2583.2000.00230.x
Keywords
heat-shock protein 90; diapause; flesh fly; Sarcophaga crassipalpis
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Heat-shock protein 23 (hsp23) and hsp70 are both known to be strongly up-regulated during pupal diapause in the flesh fly Sarcophaga crassipalpis, This prompted us to investigate whether hsp90 was also up-regulated during diapause. To test this possibility, we developed a partial clone of a hsp90 family member for use as a probe in Northern blot hybridization. Both high and low temperature exposure up-regulated hsp90 transcripts in nondiapausing individuals. In contrast to hsp23 and hsp70, hsp90 was down-regulated following entry into diapause, and returned to prediapause levels after diapause termination. The response of hsp90 to heat shock and cold shock remained intact during diapause: both shocks evoked elevated expression. The results indicate differential regulation of hsps during diapause and in response to thermal injury inflicted on diapausing pupae.
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