Journal
BIODEGRADATION
Volume 20, Issue 4, Pages 533-539Publisher
SPRINGER
DOI: 10.1007/s10532-008-9242-y
Keywords
iso-Butylparaben; n-Butylparaben; Estrogenic activity; Laccase; 1-Hydroxybenzotriazole; White rot fungus
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Funding
- [20580173]
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In the presence of a redox mediator, 1-hydroxybenzotriazole (HBT), iso-butylparaben (iso-BP) and n-butylparaben (n-BP) were treated with laccase from white rot fungus Trametes versicolor. HPLC analysis demonstrated that iso-BP and n-BP almost completely disappeared from the reaction mixture after 4 h of treatment with the laccase-HBT system. Using the yeast two-hybrid assay system, it was also confirmed that the laccase-HBT system substantially removed the estrogenic activity of iso-BP and n-BP after 4 h of treatment. Furthermore, there was a linear relationship between the removal of estrogenic activity of both parabens and the decrease in their concentrations. These results demonstrate that the laccase-HBT system is effective in eliminating iso-BP and n-BP, and removing the estrogenic activity of both parabens.
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