Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 18, Issue 4, Pages 303-309Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1026703605147
Keywords
dipolar couplings; HIV-1 Nef; ubiquitin
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The diffusive properties of biomacromolecules within the aqueous phase of polyacrylamide gels are described. High quality NMR spectra can be obtained under such conditions. As compared to water, a fivefold reduction in the translational diffusion constant, but only a 1.6-fold decrease (1.4-fold increase) in amide-N-15 T-2 (T-1) are observed for human ubiquitin within a 10% acrylamide gel. Weak alignment of the solute macromolecules can be achieved within such gels by vertical or radial compression or by the embedding of magnetically oriented purple membrane fragments. The methods are applied to derive residual dipolar couplings for human HIV-1 Nef and ubiquitin.
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