Journal
BIOCONTROL SCIENCE AND TECHNOLOGY
Volume 28, Issue 12, Pages 1128-1139Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2018.1514587
Keywords
Bacillus pumilus; Bursaphelenchus xylophilus; nematicidal substance; guanidine compound; biocontrol
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Funding
- Nature Science Foundation of the Jiangsu Higher Education Institutions of China [14KJA220002]
- Postgraduate Scientific Research and Innovation Program of Jiangsu Province [KYLX16_0859]
- National Natural Science Foundation of China [31370643]
- National Key R&D Program of China [2018YFC120003]
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Bacillus pumilus strain LYMC-3 was isolated from the stem of Pinus massoniana, and its culture filtrate exhibited strong nematicidal activity towards pinewood nematodes (Bursaphelenchus xylophilus). In this study, the nematicidal substance from the culture filtrate of B.pumilus LYMC-3 was isolated using a rotary evaporator, TLC (thin-layer chromatography), silica gel column chromatography, and HPLC. A single nematicidal compound was purified and identified as 2-{3-[(3S,8aS)-1,4-Dioxooctahydropyrrolo[1,2-a] pyrazin-3-yl] propyl}guanidine by LC-MS/MS. The median lethal concentrations (LC50) of the guanidine compound were determined as 113.5mgL(-1) and 62.5mgL(-1) against pinewood nematodes after 24h and 48h of treatment, respectively. This study demonstrates the nematicidal activity of the guanidine compound from B.pumilus LYMC-3, and highlights the high potential for the compound to be used in biocontrol of B.xylophilus.
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