Journal
BIOTECHNOLOGY LETTERS
Volume 22, Issue 24, Pages 1967-1973Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1026792430742
Keywords
hydroxyproline; 2-oxoglutarate-dependent dioxygenase; substrate specificity
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An isozyme gene of proline 3-hydroxylase was cloned from Streptomyces sp. strain TH1 (Mori H, Shibasaki T, Yano K, Ozaki A, J. Bacteriol. 1997, 179: 5677-5683). The isozyme gene (870 bp) encodes a protein of molecular weight of 33,573. Both 3-hydroxylase genes are identical at 76.2% in amino acid sequence. His-motifs conserved in 2-oxoglutarate-dependent dioxygenases are conserved in both genes. Although characteristics of both recombinant 3-hydroxylases are similar, specific activities to L-proline and proline analogs are different.
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