Journal
JOURNAL OF BIOPHOTONICS
Volume 9, Issue 3, Pages 224-234Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201400145
Keywords
Amyloid-beta; neurodegeneration; label-free; ATR-FTIR spectroscopy; surface functionalization; complex mixture; body fluid; biosensor
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Funding
- Protein Research Unit Ruhr within Europe (PURE), Ministry of Innovation, Science and Research of North-Rhine Westphalia, Germany
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The secondary structure change of the Abeta peptide to beta-sheet was proposed as an early event in Alzheimer's disease. The transition may be used for diagnostics of this disease in an early state. We present an Attenuated Total Reflection (ATR) sensor modified with a specific antibody to extract minute amounts of Abeta peptide out of a complex fluid. Thereby, the Abeta peptide secondary structure was determined in its physiological aqueous environment by FTIR-difference-spectroscopy. The presented results open the door for label-free Alzheimer diagnostics in cerebrospinal fluid or blood. It can be extended to further neurodegenerative diseases.
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