Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 409, Issue 1, Pages 31-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-2999(00)00837-2
Keywords
OAT1 (organic anion transporter 1); p-aminohippurate; methotrexate; probenecid; NSAID (nonsteroidal anti-inflammatory drug); kidney; (rat)
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The antifolate drug methotrexate is mainly eliminated from the body by renal tubular secretion via organic anion transporters. In clinical situations, severe methotrexate toxicity, due to an increase in serum concentrations, was observed after coadministration with nonsteroidal anti-inflammatory drugs (NSAIDs) or probenecid. In this study, we examined the effects of NSAIDs and probenecid on methotrexate transport via the rat renal organic anion transporter rOAT1, using Xenopus laevis oocytes. [H-3]Methotrexate uptake was markedly stimulated in the rOAT1 cRNA-injected oocytes, and this uptake was inhibited by probenecid and various NSAIDs, whereas the influence of salicylate was less. The Dixon plots showed that probenecid, indomethacin and salicylate competitively inhibited rOAT1 with apparent K-i values of 15.8 muM, 4.2, muM and 1.0 mM, respectively. These findings demonstrate that rOAT1 is the major site of the transporter-mediated interaction between methotrexate and NSAIDs and/or probenecid, leading to a decrease in renal excretion of methotrexate. (C) 2000 Elsevier Science B.V. All rights reserved.
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