Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 23, Issue 4, Pages 504-509Publisher
URBAN & FISCHER VERLAG
DOI: 10.1016/S0723-2020(00)80024-7
Keywords
16S rDNA; ARDRA; rapid identification; Lactobacillus spp.; intestinal lactic acid bacteria; PCR
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Amplified ribosomal DNA restriction analysis (ARDRA) was used to identify different species of Lactobacillus isolated from human faeces and vagina. PCR-ARDRA was performed using a set of four restriction enzymes, able to differentiate fourteen species of Lactobacillus. The PCR-ARDRA procedure described in this paper was shown to be a reliable and rapid method for identifying Lactobacillus species from intestinal and vaginal microflora at species and subspecies level.
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