Journal
INSECT MOLECULAR BIOLOGY
Volume 9, Issue 6, Pages 655-660Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2583.2000.00228.x
Keywords
carboxylesterase; gene amplification; quantitative PCR; Nilaparvata lugens
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Organophosphorus and carbamate insecticide resistance in Nilaparvata lugens is based on amplification of a carboxylesterase gene, NI-EST1. An identical gene occurs in susceptible insects. Quantitative real-time PCR was used to demonstrate that NI-EST1 is amplified 3-7-fold in the genome of resistant compared to susceptible planthoppers. Expression levels were similar to amplification levels, with 1-15-fold more NI-EST1 mRNA in individual insects and 5-11-fold more NI-EST1 mRNA in mass whole body homogenates of resistant females compared to susceptibles,These values corresponded to an 8-10-fold increase in esterase activity in the head and thorax of individual resistant insects. Although amplification, expression and activity levels of NI-EST1 in resistant N. lugens were similar, the correlation between esterase activity and NI-EST1 mRNA levels in resistant individuals was not linear.
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