Journal
BIOCONJUGATE CHEMISTRY
Volume 29, Issue 10, Pages 3394-3401Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.8b00558
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Funding
- Creative Research Initiative Grant [2014R1A3A2030423]
- Bio & Medical Technology Development Program [2012M3A9C4048780]
- Korea Institute of Science and Technology (KIST) Institutional Program [2E27870, 2E28411]
- Basic Research Program through the National Research Foundation of Korea (NRF) - Korean Government (Ministry of Science ICT) [2009-0093826, 2016R1C1B2014699]
- BK21 Plus Program
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Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with D/L-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.
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