Journal
BIOCONJUGATE CHEMISTRY
Volume 25, Issue 12, Pages 2157-2165Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc500315j
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Funding
- National Institutes of Health [T32DA022975, CA149128]
- CTSA Grant from the National Center for Research Resources (NCRR) [UL1RR024139]
- National Center for Advancing Translational Science (NCATS), components of the National Institutes of Health (NIH)
- NIH roadmap for Medical Research
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The avidin-biotin interaction permits rapid and nearly irreversible noncovalent linkage between biotinylated molecules and avidin-modified substrates. We designed a biotinylated radioligand intended for use in the detection of avidin-modified polymer nanoparticles in tissue with positron emission tomography (PET). Using an F-18 labeled prosthetic group, [F-18](4)-fluorobenzylamine, and a commercially available biotin derivate, NHS-PEG4-biotin, [F-18]-fluorobenzylamide-poly(ethylene glycol)4-biotin ([F-18]NPB4) was prepared with high purity and specific activity. The attachment of the [F-18]NPB4 radioligand to avidin-modified poly(lactic-co-glycolic acid) (PLGA) nanoparticles was tested by using PET imaging to measure the kinetics of convection-enhanced delivery (CED) of nanoparticles of varying size to the rat brain. PET imaging enabled the direct observation of nanoparticle delivery by measurement of the spatial volume of distribution of radiolabeled nanoparticles as a function of time, both during and after the infusion. This work thus validates new methods for radiolabeling PEG-biotin derivatives and also provides insight into the fate of nanoparticles that have been infused directly into the brain.
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