Journal
BIOCONJUGATE CHEMISTRY
Volume 25, Issue 7, Pages 1282-1289Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc500144u
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Funding
- Australian Research Council [DP110102183]
- Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology [CE140100036]
- National Health and Medical Research Council [APP1024723]
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Herein, the ability of porous silicon (PSi) particles for selectively binding to specific cells is investigated. PSi microparticles with a high reflectance band in the reflectivity profile are fabricated, and subsequently passivated and modified with antibodies via the Cu(I)-catalyzed alkyne-azide cycloaddition reaction and succimidyl activation. To demonstrate the ability of the antibody-modified PSI particles to selectively bind to one cell type over others, HeLa cells were transfected with surface epitopes fused to fluorescent proteins. The antibody-functionalized PSi particles showed good selectivity for the corresponding surface protein on HeLa cells, with no significant cross-reactivity. The results are important for the application of PSi particles in cell sensing and drug delivery.
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