Journal
BIOCONJUGATE CHEMISTRY
Volume 24, Issue 6, Pages 960-967Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc300677n
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Funding
- National Natural Science Foundation of China [51125014, 51233003]
- Ministry of Science and Technology of China [2011CB606202]
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Critical limb ischemia is regarded as a potentially lethal disease, and the treatment effects of existing therapies are limited. Here, in order to develop a potential approach to improve the therapy effects, we designed a peptide of TAT-PKKKRKV as the vector for VEGF(165) plasmid to facilitate in vivo angiogenesis. In in vitro studies, TAT-PKKKRKV with low cytotoxicity exhibited efficient transfection ability either with or without serum. Additionally, application of TAT-PKKKRKV/VEGF(165) complexes in hindlimb ischemia rats obviously promoted the expression of VEGF protein, which further enhanced effective angiogenesis. The results indicated that TAT-PKKKRKV is an efficient gene vector with low toxicity both in vitro and in vivo, which has great potential for clinical gene therapy.
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