Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 97, Issue 25, Pages 13537-13542Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.240460997
Keywords
calicheamicin; esperamicin; assay; molecular beacon; bleomycin
Categories
Funding
- NCI NIH HHS [P30 CA008748, R01 CA084374, CA84374, CA-08748] Funding Source: Medline
- NIGMS NIH HHS [GM58196] Funding Source: Medline
Ask authors/readers for more resources
Although extensive effort has been applied toward understanding the mechanism by which enediynes cleave DNA, a continuous assay for this phenomenon is still lacking. In fact, with the exception of assays for DNase, continuous assays for most DNA cleavage events are unavailable. This article describes the application of molecular break lights (a single-stranded oligonucleotide that adopts a stem-and-loop structure and carries a 5' fluorescent moiety, a 3'-nonfluorescent quenching moiety, and an appropriate cleavage site within the stem) to develop the first continuous assay for cleavage of DNA by enediynes. Furthermore, the generality of this approach is demonstrated by using the described assay to directly compare the DNA cleavage by naturally occurring enediynes [calicheamicin and esperamicin), non-enediyne small molecule agents (bleomycin, methidiumpropyl-EDTA-Fe(lt). and EDTA-Fe(II]). as well as the restriction endonuclease BamHI. Given the simplicity, speed, and sensitivity of this approach, the described methodology could easily be extended to a high throughput format and become a new method of choice in modern drug discovery to screen for novel protein-based or small molecule-derived DNA cleavage agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available