Journal
BIOCONJUGATE CHEMISTRY
Volume 22, Issue 2, Pages 264-273Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc1003923
Keywords
-
Categories
Funding
- Spanish Ministry of Science and Innovation [CTQ2008-04638]
- Department of Industry of the Basque Country [ETORTEK]
- CIBER-BBN
- CICbiomaGUNE
Ask authors/readers for more resources
A versatile nanoplatform based on magnetic glyconanoparticles (glyco-ferrites) to attach well-oriented antibodies is described. An efficient ligand exchange process has been used to prepare water-soluble 6-nm-sized core shell Fe3O4@Au nanoparticles bearing amphiphilic carbohydrates and aliphatic ethylene glycol chains ended in a carboxyl group. The covalent immobilization through the carboxyl group of an Fc receptor (protein G) enables successful well-oriented capture of immunoglobulins G onto the magnetic glyconanoparticle. A thorough characterization of structure and biofunctionality of the constructs is carried out by different techniques. The selective immunolabeling of cells by the antibody-magnetic glyconanoparticle conjugates is demonstrated by magnetic resonance imaging (MRI), as well as by fluorescence techniques.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available