Journal
JOURNAL OF CELL BIOLOGY
Volume 151, Issue 6, Pages 1337-1343Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.151.6.1337
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Funding
- NIGMS NIH HHS [R01 GM047337, R01 GM047337-09, GM47337] Funding Source: Medline
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In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dyneindependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.
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