Journal
BIOCONJUGATE CHEMISTRY
Volume 22, Issue 10, Pages 1916-1923Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc200083p
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- MESR
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Cationic surfactants easily interact with plasmid DNA to form small lipoplexes. However, their detergent behavior and associated biological toxicity limit their use as gene delivery vectors. We have incorporated a diacetylene motif in the hydrophobic chain of cationic surfactants. By using UV irradiation, the small cationic micelles (9 nm) obtained with diacetylenic detergents were photo-olymerized into 40 nm spheres. Electrostatic interactions with plasmnid DNA led to the formation of 45 nm lipoplexes at N/P = 5 ratio. In vitro transfection of the pCMV-Luciferase plasmid resulted in gene expression (>10(10) RLU/mg protein) at the same ratio, comparable with the commercially available JetSi-ENDO gene delivery system. This new and versatile class of molecules could lead to a new generation of in vivo gene delivery vectors.
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