4.8 Article

DNA damage-induced phosphorylation of p53 at serine 20 correlates with p21 and Mdm-2 induction in vivo

Journal

ONCOGENE
Volume 19, Issue 54, Pages 6203-6208

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1204017

Keywords

phosphorylation; ionizing radiation; p53; p21

Funding

  1. NCI NIH HHS [CA77339, CA16672, CA67987, CA55164] Funding Source: Medline

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We investigated the induction and physiological role of Ser20 phosphorylation of p53 in response to DNA damage caused by ionizing radiation (IR) or ultraviolet radiation (UV), A polyclonal antibody that specifically recognizes a p53 peptide containing phosphorylated Ser20 was generated and used to detect p53 phosphorylation at Ser20, Western blot analyses of p53 in four cell lines with this antibody revealed that the p53 protein was phosphorylated at Ser20 to a different extent after treatment with IR or UV, The phosphorylation of Ser20 of wild-type p53 correlated with enhanced induction of the p53 downstream target genes p21(WAF1/Cip1) (p21) and mdm-2. These results suggest that DNA damage-induced phosphorylation of p53 at Ser20 enhances the transactivation function of p53 for p21 and mdm-2 in vivo.

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