Journal
BIOCONJUGATE CHEMISTRY
Volume 21, Issue 1, Pages 182-186Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc900316q
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Funding
- MEXT, Japan [19681023]
- RIKEN Chemical Biology Project
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The introduction of a cleavable site in it photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule oil the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.
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