NADPH oxidase activity is required for endothelial cell proliferation and migration

NADPH oxidase has been shown to play an important role in cardiovascular biology. The goal of the present study,vas to determine whether NADPH oxidase activity is important for endothelial cell growth and migration. In proliferation assays, growth factor- or serum-induced DNA synthesis in three different types of human endothelial cells was abrogated by inhibitors of NADPH oxidase, but not by inhibitors of xanthine oxidase or nitric oxide synthase, Moreover, vascular endothelial growth factor-induced migration of human endothelial cells mas suppressed in the presence of NADPH oxidase inhibitors. These results support a potential role for NADPH oxidase in mediating angiogenesis. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.