Journal
ANALYTICAL CHEMISTRY
Volume 72, Issue 24, Pages 5918-5924Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0006167
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We describe herein fluorescent indicators for cyclic GMP (cGMP) in single living cells. cGMP-dependent protein kinase I alpha (PKG I alpha), a receptor for cGMP, was fused with blue- and red-shifted green fluorescent proteins (GFPs) to its N- and C-termini, respectively. Using PKG I alpha (Delta1-47), in which the dimerization domain was deleted, fluorescence resonance energy transfer between the GFPs was found to increase upon cGMP-induced conformational change in PKG I alpha (Delta1-47) We demonstrated that thus developed fluorescent indicators reversibly responded to cGMP that was produced in nitric oxide-stimulated HEK293 cells. The present genetically encoded fluorescent indicators open a way not only for understanding the dynamics of cGMP signaling in single cells and organisms but also for discovering pharmaceuticals such as isoform-specific inhibitors for phosphodiesterases.
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