Journal
BIOCONJUGATE CHEMISTRY
Volume 21, Issue 11, Pages 2065-2075Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc100288c
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Funding
- National Institutes of Health [GM-069862]
- National Science Foundation [CHE-0243496]
- Department of Defense [W911SR-08-C-0001]
- Center for Sensing Science and Technology at Purdue University
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Bishydrazides are versatile linkers for attaching glycans to substrates for lectin binding and pathogen detection schemes. The alpha,omega-bishydrazides of carboxymethylated hexa(ethylene glycol) (4) can be conjugated at one end to unprotected oligosaccharides, then attached onto carrier proteins, tethered onto activated carboxyl-terminated surfaces, or functionalized with a photoactive cross-linking agent for lithographic patterning. Glycoconjugates of bishydrazide 4 can also be converted into dithiocarbamates (DTCs) by treatment with CS2 under mild conditions, for attachment onto gold substrates. The immobilized glycans serve as recognition elements for cell-surface lectins and enable the detection and capture of bacterial pathogens such as Pseudomonas aeruginosa by their adsorption onto micropatterned substrates. A detection limit of 10(3) cfu/mL is demonstrated, using a recently introduced method based on optical pattern recognition.
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